{"product_id":"proteintech-85760-4-rr","title":"Proteintech, 85760-4-RR, RIPK2 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe RIPK2 (85760-4-RR) by Proteintech is a Recombinant antibody targeting RIPK2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n85760-4-RR targets RIPK2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293T cells,  THP-1 cells,  Ramos cells,  Raji cells,  Jurkat cells,  EL-4 cells\nPositive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Jurkat cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine\/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag0909 Product name: Recombinant human RIPK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 221-531 aa of BC004553 Sequence: EVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPS Predict reactive species\nFull Name: receptor-interacting serine-threonine kinase 2\nCalculated Molecular Weight: 61 kDa\nObserved Molecular Weight: 46-55 kDa, 61-72 kDa\nGenBank Accession Number: BC004553\nGene Symbol: RIPK2\nGene ID (NCBI): 8767\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: O43353\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888815100073,"sku":"85760-4-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-85760-4-rr","provider":"Iright","version":"1.0","type":"link"}