Proteintech, 85886-4-RR, WNK4 Recombinant monoclonal antibody

Size: 20ul / 100ul The WNK4 (85886-4-RR) by Proteintech is a Recombinant antibody targeting WNK4 in WB, ELISA applications with reactivity to human samples 85886-4-RR targets WNK4 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, A375 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information WNK4 (With No Lysine Kinase 4) is a member of the WNK family of serine/threonine protein kinases. It plays a crucial role in regulating electrolyte balance and blood pressure by modulating the activity of ion transporters in the kidney. WNK4 is primarily involved in the regulation of sodium and potassium homeostasis. It achieves this by phosphorylating and activating the kinases SPAK and OSR1, which in turn regulate the activity of the sodium-chloride cotransporter (NCC) in the distal convoluted tubule of the kidney. Additionally, by promoting sodium reabsorption and potassium secretion, WNK4 contributes to the regulation of blood pressure. Increased activity of WNK4 can lead to hypertension. (PMID: 24655003; 33491560) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag17773 Product name: Recombinant human WNK4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 845-1243 aa of BC136664 Sequence: PISSQVSSNPSPHPTSSPLPFSSSTPEFPVPLSQCPWSSLPTTSPPTFSPTCSQVTLSSPFFPPCPSTSSFPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPSPLPSLPLPPPVAPGGQESPSPHTAEVESEASPPPARPLPGEARLAPISEEGKPQLVGRFQVTSSKEPAEPLPLQPTSPTLSGSPKPSTPQLTSESSDTEDSAGGGPETREALAESDRAAEGLGAGVEEEGDDGKEPQVGGSPQPLSHPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQSLRQKHLSEVETLQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM Predict reactive species Full Name: WNK lysine deficient protein kinase 4 Calculated Molecular Weight: 1243 aa, 135 kDa Observed Molecular Weight: 220 kDa GenBank Accession Number: BC136664 Gene Symbol: WNK4 Gene ID (NCBI): 65266 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96J92 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.