Proteintech, 85929-5-RR, TH Recombinant monoclonal antibody

Size: 20ul / 100ul The TH (85929-5-RR) by Proteintech is a Recombinant antibody targeting TH in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 85929-5-RR targets TH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat brain tissue, PC-12 cells, mouse brain tissue, fetal human brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: rat brain tissue, mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:1250-1:5000 Immunofluorescence (IF)-P: IF-P : 1:500-1:2000 Background Information TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag23034 Product name: Recombinant human TH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC104967 Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER Predict reactive species Full Name: tyrosine hydroxylase Calculated Molecular Weight: 528 aa, 59 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC104967 Gene Symbol: TH Gene ID (NCBI): 7054 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P07101 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.