Proteintech, 86050-1-RR, SERCA2/ATP2A2 Recombinant monoclonal antibody

Size: 20ul / 100ul The SERCA2/ATP2A2 (86050-1-RR) by Proteintech is a Recombinant antibody targeting SERCA2/ATP2A2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86050-1-RR targets SERCA2/ATP2A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: 37°C incubated mouse heart tissue, 37℃ incubated mouse heart, rat heart, 37℃ incubated rat heart Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: C2C12 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag26177 Product name: Recombinant human SERCA2,ATP2A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 314-580 aa of BC035588 Sequence: VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDS Predict reactive species Full Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 Calculated Molecular Weight: 1042 aa, 115 kDa Observed Molecular Weight: 110-115 kDa GenBank Accession Number: BC035588 Gene Symbol: SERCA2 Gene ID (NCBI): 488 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P16615 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.