Proteintech, 86120-2-RR, PSMD10/Gankyrin Recombinant monoclonal antibody

Size: 20ul / 100ul The PSMD10/Gankyrin (86120-2-RR) by Proteintech is a Recombinant antibody targeting PSMD10/Gankyrin in WB, IHC, ELISA applications with reactivity to human samples 86120-2-RR targets PSMD10/Gankyrin in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, PC-3 cells, K-562 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information PSMD10, also known as Gankyrin, is a subunit of the 19S regulatory particle of the proteasome and plays a crucial role in protein degradation. It is a small, 25 kDa cytoplasmic-nuclear shuttling protein, highly conserved throughout evolution (~40% identity to yeast Nas6p). PSMD10 is involved in the regulation of cell cycle progression and apoptosis by interacting with key proteins such as p53 and Rb. PSMD10 has been identified as an oncoprotein, promoting tumor cell proliferation and survival in various cancers (PMID: 16581249, PMID: 31576540). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2994 Product name: Recombinant human PSMD10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 4-226 aa of BC011960 Sequence: CVSNLMVCNLAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG Predict reactive species Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 Calculated Molecular Weight: 226 aa, 24 kDa Observed Molecular Weight: 24-28 kDa GenBank Accession Number: BC011960 Gene Symbol: PSMD10 Gene ID (NCBI): 5716 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O75832 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.