Proteintech, 86138-1-RR, SPINT1/HAI-1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SPINT1/HAI-1 (86138-1-RR) by Proteintech is a Recombinant antibody targeting SPINT1/HAI-1 in WB, ELISA applications with reactivity to human, mouse samples 86138-1-RR targets SPINT1/HAI-1 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: T-47D cells, MCF-7 cells, mouse small intestine tissue, mouse colon tissue, Daudi cells, human placenta Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg3131 Product name: Recombinant Human SPINT1 protein (rFc Tag) (HPLC verified) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 35-465 aa of NM_181642.2 Sequence: AGPPPAPPGLPAGADCLNSFTAGVPGFVLDTNASVSNGATFLESPTVRRGWDCVRACCTTQNCNLALVELQPDRGEDAIAACFLINCLYEQNFVCKFAPREGFINYLTREVYRSYRQLRTQGFGGSGIPKAWAGIDLKVQPQEPLVLKDVENTDWRLLRGDTDVRVERKDPNQVELWGLKEGTYLFQLTVTSSDHPEDTANVTVTVLSTKQTEDYCLASNKVGRCRGSFPRWYYDPTEQICKSFVYGGCLGNKNNYLREEECILACRGVQGGPLRGSSGAQATFPQGPSMERRHPVCSGTCQPTQFRCSNGCCIDSFLECDDTPNCPDASDEAACEKYTSGFDELQRIHFPSDKGHCVDLPDTGLCKESIPRWYYNPFSEHCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREIPIPSTGSVE Predict reactive species Full Name: serine peptidase inhibitor, Kunitz type 1 Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 58-70 kDa GenBank Accession Number: NM_181642.2 Gene Symbol: HAI-1 Gene ID (NCBI): 6692 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O43278-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.