{"product_id":"proteintech-86307-1-rr","title":"Proteintech, 86307-1-RR, Phospho-Cofilin (Ser3) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-Cofilin (Ser3) (86307-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Cofilin (Ser3) in WB, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples\n86307-1-RR targets Phospho-Cofilin (Ser3) in WB, IF\/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, NIH\/3T3 cells,  λ phosphatase treated HeLa cells,  λ phosphatase treated NIH\/3T3 cells\nPositive IF\/ICC detected in: λ phosphatase treated Hela cells\nPositive FC (Intra) detected in: λ phosphatase treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)\/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: cofilin 1 (non-muscle)\nCalculated Molecular Weight: 18 kDa\nObserved Molecular Weight: 18 kDa\nGenBank Accession Number: BC012318\nGene Symbol: Cofilin\nGene ID (NCBI): 1072\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P23528\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888655814825,"sku":"86307-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-86307-1-rr","provider":"Iright","version":"1.0","type":"link"}