Proteintech, 86315-2-RR, PRPF31 Recombinant monoclonal antibody

Size: 20ul / 100ul The PRPF31 (86315-2-RR) by Proteintech is a Recombinant antibody targeting PRPF31 in WB, IF/ICC, ELISA applications with reactivity to human samples 86315-2-RR targets PRPF31 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, A549 cells, Caco-2 cells, OVCAR-3 cells Positive IF/ICC detected in: Jurkat cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag26716 Product name: Recombinant human PRPF31 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 101-212 aa of NM_015629 Sequence: MVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRM Predict reactive species Full Name: PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 55-60 kDa GenBank Accession Number: NM_015629 Gene Symbol: PRPF31 Gene ID (NCBI): 26121 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8WWY3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.