Proteintech, 86323-1-RR, PLS3 Recombinant monoclonal antibody

Size: 20ul / 100ul The PLS3 (86323-1-RR) by Proteintech is a Recombinant antibody targeting PLS3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 86323-1-RR targets PLS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, COLO 320 cells, MCF-7 cells, HEK-293 cells, A431 cells, NIH/3T3 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PLS3, also named T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. L-plastin plays an important role in leukocyte function, and T-plastin is possibly involved in DNA repair. Both T- and L-plastin are implicated in several diseases, and L-plastin is considered to be a valuable marker for cancer (PMID: 15960882). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: plastin 3 (T isoform) Calculated Molecular Weight: 71 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: NM_005032 Gene Symbol: PLS3 Gene ID (NCBI): 5358 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P13797 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.