Proteintech, 86840-3-RR, TOM70 Recombinant monoclonal antibody

Size: 20ul / 100ul The TOM70 (86840-3-RR) by Proteintech is a Recombinant antibody targeting TOM70 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86840-3-RR targets TOM70 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MDA-MB-231 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6027 Product name: Recombinant human TOM70 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 245-608 aa of BC052994 Sequence: KNREPLMPSPQFIKSYFSSFTDDIISQPMLKGEKSDEDKDKEGEALEVKENSGYLKAKQYMEEENYDKIISECSKEIDAEGKYMAEALLLRATFYLLIGNANAAKPDLDKVISLKEANVKLRANALIKRGSMYMQQQQPLLSTQDFNMAADIDPQNADVYHHRGQLKILLDQVEEAVADFDECIRLRPESALAQAQKCFALYRQAYTGNNSSQIQAAMKGFEEVIKKFPRCAEGYALYAQALTDQQQFGKADEMYDKCIDLEPDNATTYVHKGLLQLQWKQDLDRGLELISKAIEIDNKCDFAYETMGTIEVQRGNMEKAIDMFNKAINLAKSEMEMAHLYSLCDAAHAQTEVAKKYGLKPPTL Predict reactive species Full Name: translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae) Calculated Molecular Weight: 67 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC052994 Gene Symbol: TOM70 Gene ID (NCBI): 9868 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O94826 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.