{"product_id":"proteintech-86847-1-rr","title":"Proteintech, 86847-1-RR, VAT1 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe VAT1 (86847-1-RR) by Proteintech is a Recombinant antibody targeting VAT1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n86847-1-RR targets VAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: SH-SY5Y cells, NIH\/3T3 cells,  C6 cells,  mouse brain tissue,  rat brain tissue,  Jurkat cells,  MCF-7 cells\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nVesicle amine transport protein 1 (VAT1) is expressed in neurons and neuroendocrine cells, where it participates in vesicular monoamine uptake and in the docking\/fusion steps of neurotransmitter release. Beyond the nervous system, VAT1 binds anionic phospholipids (phosphatidic acid and phosphatidylserine) and catalyses their inter-membrane transfer, thereby integrating lipid metabolism with vesicle trafficking. Recent tumour-biology studies have revealed that VAT1 is frequently up-regulated in aggressive gliomas and gastric cancers; its abundance correlates with enhanced cell migration, radioresistance and an immunosuppressive micro-environment. (PMID: 39832459,PMID: 31524269)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag16652 Product name: Recombinant human VAT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-393 aa of BC015041 Sequence: MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN Predict reactive species\nFull Name: vesicle amine transport protein 1 homolog (T. californica)\nCalculated Molecular Weight: 42 kDa\nObserved Molecular Weight: 45 kDa\nGenBank Accession Number: BC015041\nGene Symbol: VAT1\nGene ID (NCBI): 10493\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q99536\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888840560809,"sku":"86847-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-86847-1-rr","provider":"Iright","version":"1.0","type":"link"}