Proteintech, 86992-1-RR, Tri-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody

Size: 20ul / 100ul The Tri-Methyl-Histone H3 (Lys27) (86992-1-RR) by Proteintech is a Recombinant antibody targeting Tri-Methyl-Histone H3 (Lys27) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human samples 86992-1-RR targets Tri-Methyl-Histone H3 (Lys27) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293T cells, K-562 cells Positive IF/ICC detected in: HeLa cells Positive Dot Blot detected in: / / Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 DOT BLOT: DOT BLOT : 1:10-1:100 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information H3K27me3 is a typical transcriptional repression marker that primarily silences key developmental genes through synergistic action with polycomb group proteins. In embryonic stem cells, many developmental regulatory genes are marked by H3K27me3 and remain silent; during cell differentiation, the H3K27me3 markers for specific genes are removed, thereby activating the corresponding developmental programs. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 15-17 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.