{"product_id":"proteintech-86992-1-rr","title":"Proteintech, 86992-1-RR, Tri-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Tri-Methyl-Histone H3 (Lys27) (86992-1-RR) by Proteintech is a Recombinant antibody targeting Tri-Methyl-Histone H3 (Lys27) in WB, IF\/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human samples\n86992-1-RR targets Tri-Methyl-Histone H3 (Lys27) in WB, IF\/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293T cells,  K-562 cells\nPositive IF\/ICC detected in: HeLa cells\nPositive Dot Blot detected in: \/ \/\nPositive ChIP-qPCR detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:500-1:2000\nDOT BLOT: DOT BLOT : 1:10-1:100\nCHIP-QPCR: CHIP-QPCR : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nH3K27me3 is a typical transcriptional repression marker that primarily silences key developmental genes through synergistic action with polycomb group proteins. In embryonic stem cells, many developmental regulatory genes are marked by H3K27me3 and remain silent; during cell differentiation, the H3K27me3 markers for specific genes are removed, thereby activating the corresponding developmental programs.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: histone cluster 1, H3a\nObserved Molecular Weight: 15-17 kDa\nGenBank Accession Number: BC066245\nGene Symbol: HIST1H3A\nGene ID (NCBI): 8350\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P68431\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888836628649,"sku":"86992-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-86992-1-rr","provider":"Iright","version":"1.0","type":"link"}