Product Description
Size: 20ul / 100ul
The PRKCSH (87004-3-RR) by Proteintech is a Recombinant antibody targeting PRKCSH in WB, ELISA applications with reactivity to human, rat samples
87004-3-RR targets PRKCSH in WB, ELISA applications and shows reactivity with human, rat samples.
Tested Applications
Positive WB detected in: HeLa cells, Jurkat cells, HEK-293T cells, Daudi cells, rat liver tissue
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Background Information
PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.
Specification
Tested Reactivity: human, rat
Host / Isotype: Rabbit / IgG
Class: Recombinant
Type: Antibody
Immunogen: CatNo: Ag2796 Product name: Recombinant human PRKCSH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 157-506 aa of BC013586 Sequence: TVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYRSEALPTDLPTPSAPDLTEPKEEQPPVPSSPTEEEEEEEEEEEEAEEEEEEEDSEEAPPPLSPPQPASPAEEDKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL Predict reactive species
Full Name: protein kinase C substrate 80K-H
Calculated Molecular Weight: 80 kDa
Observed Molecular Weight: 80 kDa
GenBank Accession Number: BC013586
Gene Symbol: PRKCSH
Gene ID (NCBI): 5589
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein A purification
UNIPROT ID: P14314
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
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