{"product_id":"proteintech-87086-1-rr","title":"Proteintech, 87086-1-RR, RNF6 Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe RNF6 (87086-1-RR) by Proteintech is a Recombinant antibody targeting RNF6 in WB, ELISA applications with reactivity to human, rat samples\n87086-1-RR targets RNF6 in WB, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: PC-3 cells, SKOV-3 cells,  Sp2\/0 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag14209 Product name: Recombinant human RNF6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC034688 Sequence: MNQSRSRSDGGSEETLPQDHNHHENERRWQQERLHREEAYYQFINELNDEDYRLMRDHNLLGTPGEITSEELQQRLDGVKEQLASQPDLRDGTNYRDSEVPRESSHEDSLLEWLNTFRRTGNATRSGQNGNQTWRAVSRTNPNNGEFRFSLEIHVNHENRGFEIHGEDYTDIPLSDSNRDHTANRQQRSTSPVARRTRSQTSVNFNGSSSNIPRTRLASRGQNPAEGSFSTLGRLRNGIGGAAGIPRANASRTNFSSHTNQSGGSELRQREGQRFGAAHVWENGARSNVTVRNTNQRLEP Predict reactive species\nFull Name: ring finger protein (C3H2C3 type) 6\nCalculated Molecular Weight: 685 aa, 78 kDa\nObserved Molecular Weight: 75-78 kDa\nGenBank Accession Number: BC034688\nGene Symbol: RNF6\nGene ID (NCBI): 6049\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q9Y252\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888816181417,"sku":"87086-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-87086-1-rr","provider":"Iright","version":"1.0","type":"link"}