{"product_id":"proteintech-87088-1-rr","title":"Proteintech, 87088-1-RR, Phospho-MYPT1 (Thr853) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-MYPT1 (Thr853) (87088-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-MYPT1 (Thr853) in WB, ELISA applications with reactivity to human, rat samples\n87088-1-RR targets Phospho-MYPT1 (Thr853) in WB, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  C6 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMyosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Phospho-MYPT1 (Thr853) is a phosphorylated form of MYPT1 (myosin phosphatase target subunit 1) at threonine 853. This phosphorylation event is associated with the regulation of smooth muscle contraction and cell motility. The Rho-associated protein kinase (ROCK) is known to phosphorylate MYPT1 at Thr853, which in turn suppresses the activity of myosin light chain phosphatase (MLCP), leading to an increase in myosin light chain phosphorylation and subsequent smooth muscle contraction. (PMID: 24712327)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: protein phosphatase 1, regulatory (inhibitor) subunit 12A\nCalculated Molecular Weight: 1030 aa, 115 kDa\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: BC111752\nGene Symbol: MYPT1\nGene ID (NCBI): 4659\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: O14974\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888803369129,"sku":"87088-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-87088-1-rr","provider":"Iright","version":"1.0","type":"link"}