Proteintech, 87266-1-RR, ZO-3 Recombinant monoclonal antibody

Size: 20ul / 100ul The ZO-3 (87266-1-RR) by Proteintech is a Recombinant antibody targeting ZO-3 in WB, IP, ELISA applications with reactivity to human samples 87266-1-RR targets ZO-3 in WB, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HT-29 cells, HCT 116 cells, Caco-2 cells Positive IP detected in: HT-29 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg5585 Product name: Recombinant human ZO3/Zonula occludens PROTEIN 3 protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 835-919 aa of NM_001267560.1 Sequence: DEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL Predict reactive species Full Name: tight junction protein 3 (zona occludens 3) Calculated Molecular Weight: 101 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: NM_001267560.1 Gene Symbol: ZO-3 Gene ID (NCBI): 27134 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O95049-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.