Proteintech, 87269-1-RR, STK3/MST2 Recombinant monoclonal antibody

Size: 20ul / 100ul The STK3/MST2 (87269-1-RR) by Proteintech is a Recombinant antibody targeting STK3/MST2 in WB, ELISA applications with reactivity to human samples 87269-1-RR targets STK3/MST2 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information The protein encoded by STK3 gene is a key serine/threonine kinase, and its protein product can be cleaved by caspase in the process of apoptosis, and the inhibitory C-terminal part is removed, and the N-terminal part is transferred to the nucleus to form homodimer, which becomes an active kinase and promotes chromatin condensation in the process of apoptosis. STK3 is one of the core components of Hippo signaling pathway, which plays a key role in controlling cell proliferation, apoptosis and tissue growth. In the Hippo signaling pathway, STK3 forms a cascade reaction with STK4, LATS1/2 and other kinases, phosphorylates and activates LATS1/2, and then inhibits transcription coactivators YAP and TAZ, thus controlling cell growth and preventing cancer progression. STK3 and STK4(MST1) are similar in structure and function. They have conserved amino-terminal kinase domains, carboxyl-terminal regulatory domains and interaction domains. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2741 Product name: Recombinant human STK3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC010640 Sequence: MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQ Predict reactive species Full Name: serine/threonine kinase 3 (STE20 homolog, yeast) Calculated Molecular Weight: 491 aa, 56 kDa Observed Molecular Weight: 56-63 kDa GenBank Accession Number: BC010640 Gene Symbol: STK3 Gene ID (NCBI): 6788 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13188 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.