{"product_id":"proteintech-87337-1-rr","title":"Proteintech, 87337-1-RR, Phospho-CAD (Ser1859) Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Phospho-CAD (Ser1859) (87337-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-CAD (Ser1859) in WB, ELISA applications with reactivity to human samples\n87337-1-RR targets Phospho-CAD (Ser1859) in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  λ phosphatase treated HEK-293 cells,  λ phosphatase treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides. Phospho-CAD (Ser1859) is a phosphorylated form of the CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) enzyme at Serine 1859. This site is specifically phosphorylated by mTOR complex 1 (mTORC1), acting through the downstream kinase S6K1, and plays a role in regulating pyrimidine biosynthesis. The phosphorylation of CAD at Ser1859 is a part of the PI3K-AKT-mTORC1-S6K1 pathway that integrates growth signals to regulate cell growth, proliferation, and homeostasis by modulating several anabolic and catabolic processes.In addition, insulin also stimulates the Ser1859 phosphorylation of CAD in a manner sensitive to rapamycin as well as phosphatase and tensin homolog (PTEN) deletion in the PI3K-AKT pathway. (PMID:25422319, PMID: 34638594)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase\nCalculated Molecular Weight: 2225 aa, 243 kDa\nObserved Molecular Weight: 250 kDa\nGenBank Accession Number: NM_004341\nGene Symbol: CAD\nGene ID (NCBI): 790\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P27708\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888799961257,"sku":"87337-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-87337-1-rr","provider":"Iright","version":"1.0","type":"link"}