{"product_id":"proteintech-87431-1-rr","title":"Proteintech, 87431-1-RR, HMBS Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe HMBS (87431-1-RR) by Proteintech is a Recombinant antibody targeting HMBS in WB, ELISA applications with reactivity to human samples\n87431-1-RR targets HMBS in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  HepG2 cells,  Caco-2 cells,  L02 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHydroxymethylbilane synthase (HMBS), also known as porphobilinogen deaminase (PBGD) or previously as uroporphyrinogen I synthase, is a key enzyme in the heme biosynthesis pathway. HMBS is a cytoplasmic enzyme that catalyzes a critical step in the heme biosynthesis pathway: the polymerization of four porphobilinogen molecules to form linear hydroxymethylbilane. It is expressed in all tissues, with the highest levels observed in the liver and erythroid precursors to meet high heme demand. Two main isoforms exist: a ubiquitous \"housekeeping\" isoform and an erythroid-specific isoform. The observed molecular weight of human HMBS protein is approximately 40-42 kDa (as determined by SDS-PAGE). The erythroid-specific isoform has a slightly lower molecular weight due to a different translation initiation site. (PMID: 19292878, PMID: 12555854)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag6509 Product name: Recombinant human HMBS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC000520 Sequence: MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH Predict reactive species\nFull Name: hydroxymethylbilane synthase\nCalculated Molecular Weight: 39 kDa\nObserved Molecular Weight: 42 kDa\nGenBank Accession Number: BC000520\nGene Symbol: HMBS\nGene ID (NCBI): 3145\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: P08397\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888761917609,"sku":"87431-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-87431-1-rr","provider":"Iright","version":"1.0","type":"link"}