Proteintech, 87453-1-RR, RAB25 Recombinant monoclonal antibody

Size: 20ul / 100ul The RAB25 (87453-1-RR) by Proteintech is a Recombinant antibody targeting RAB25 in WB, ELISA applications with reactivity to human samples 87453-1-RR targets RAB25 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HT-29 cells, Caco-2 cells, T-47D cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information RAB25 (RAB25, member RAS oncogene family), also known as RAB11C or CATX-8, is a small GTPase belonging to the RAS superfamily of proteins. RAB25 is a key regulator of receptor recycling and polarized vesicular trafficking in epithelial cells. Unlike many RAB proteins, RAB25 expression is predominantly restricted to epithelial tissues and is frequently dysregulated in carcinomas. It functions as a critical determinant of cellular fate through its context-dependent roles in modulating major signaling pathways, including integrin recycling, growth factor receptor trafficking (e.g., EGFR, IGF1R), and autophagy. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg5536 Product name: Recombinant human RAB25 protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 1-210 aa of NM_020387.3 Sequence: MGNGTEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAVKAQIWDTAGLERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQAREVPTEEARMFAENNGLLFLETSALDSTNVELAFETVLKEIFAKVSKQRQNSIRTNAITLGSAQAGQEPGPGEKRACC Predict reactive species Full Name: RAB25, member RAS oncogene family Calculated Molecular Weight: 23 kDa Observed Molecular Weight: 24 kDa GenBank Accession Number: NM_020387.3 Gene Symbol: RAB25 Gene ID (NCBI): 57111 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P57735 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.