{"product_id":"proteintech-87471-1-rr","title":"Proteintech, 87471-1-RR, Nicastrin Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe Nicastrin (87471-1-RR) by Proteintech is a Recombinant antibody targeting Nicastrin in WB, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n87471-1-RR targets Nicastrin in WB, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, Neuro-2a cells,  HEK293-T cells,  U87 MG cells,  SH-SY5Y cells,  Jurkat cells,  C6 cells\nPositive IF\/ICC detected in: C6 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease that cleaves many type I membrane proteins, including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytic processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag5137 Product name: Recombinant human Nicastrin protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 338-689 aa of BC047621 Sequence: NVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIHARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY Predict reactive species\nFull Name: nicastrin\nCalculated Molecular Weight: 709 aa, 78.4 kDa\nObserved Molecular Weight: 110 kDa, 130 kDa\nGenBank Accession Number: BC047621\nGene Symbol: Nicastrin\nGene ID (NCBI): 23385\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q92542\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888790261929,"sku":"87471-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-87471-1-rr","provider":"Iright","version":"1.0","type":"link"}