Proteintech, 87550-1-RR, TBP Recombinant monoclonal antibody

Size: 100ul The TBP (87550-1-RR) by Proteintech is a Recombinant antibody targeting TBP in WB, ELISA applications with reactivity to human samples 87550-1-RR targets TBP in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, PC-3 cells, A2780 cells, COLO 320 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information TBP, TATA box-binding protein, is a DNA-binding subunit of TFIID, which is a multisubunit complex essential for the expression of most protein-encoding genes. The well-conserved C-terminal domains of 180 aa is both necessary and sufficient for interaction with DNA and for assembly of the basal transcription apparatus.The N terminus of TBP modulates the DNA-binding activity of the C terminus of the protein. Once binds to the TATA box mediated by TBP, TFIID initiate the transcription step of the pre-initiation complex(PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag12383 Product name: Recombinant human TBP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-338 aa of BC110341 Sequence: MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT Predict reactive species Full Name: TATA box binding protein Calculated Molecular Weight: 338 aa, 38 kDa Observed Molecular Weight: 37-45 kDa GenBank Accession Number: BC110341 Gene Symbol: TBP Gene ID (NCBI): 6908 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P20226 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.