Proteintech, 87558-1-RR, TOP3A Recombinant monoclonal antibody

Size: 20ul / 100ul The TOP3A (87558-1-RR) by Proteintech is a Recombinant antibody targeting TOP3A in WB, ELISA applications with reactivity to human, mouse, rat samples 87558-1-RR targets TOP3A in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, A549 cells, HCT 116 cells, mouse testis tissue, rat testis tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. This antibody is specific to react with the 110 kDa human TOP3A. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6010 Product name: Recombinant human TOP3A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 652-1001 aa of BC051748 Sequence: PEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR Predict reactive species Full Name: topoisomerase (DNA) III alpha Calculated Molecular Weight: 1001 aa, 112 kDa Observed Molecular Weight: 100~110 kDa GenBank Accession Number: BC051748 Gene Symbol: TOP3A Gene ID (NCBI): 7156 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13472 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.