{"product_id":"proteintech-87558-1-rr","title":"Proteintech, 87558-1-RR, TOP3A Recombinant monoclonal antibody","description":"Size: 20ul \/ 100ul\nThe TOP3A (87558-1-RR) by Proteintech is a Recombinant antibody targeting TOP3A in WB, ELISA applications with reactivity to human, mouse, rat samples\n87558-1-RR targets TOP3A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  MCF-7 cells,  A549 cells,  HCT 116 cells,  mouse testis tissue,  rat testis tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. This antibody is specific to react with the 110 kDa human TOP3A.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: CatNo: Ag6010 Product name: Recombinant human TOP3A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 652-1001 aa of BC051748 Sequence: PEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR Predict reactive species\nFull Name: topoisomerase (DNA) III alpha\nCalculated Molecular Weight: 1001 aa, 112 kDa\nObserved Molecular Weight: 100~110 kDa\nGenBank Accession Number: BC051748\nGene Symbol: TOP3A\nGene ID (NCBI): 7156\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: Q13472\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888834269353,"sku":"87558-1-RR","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-87558-1-rr","provider":"Iright","version":"1.0","type":"link"}