Proteintech, CL555-tgt, ChromoTek anti-TIGIT recombinant VHH, CoraLite® Plus 555

Size: 150ul CL555-tgt targets TIGIT in FC and IF applications and shows reactivity with Human samples. Specification Applications: FC, IF Reactivity: Human Conjugate: CoraLite® Plus 555 Type: Nanobody Class: Recombinant Host: Alpaca Affinity: Picomolar range, below the assay limit (biolayer interferometry) Molecular Weight: 14.346 kDa Form: Liquid Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm RRID: AB_3101925 Storage Buffer: 500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide Storage Condition: Store at -20°C; Shipped at ambient temperature. Background: TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).