{"product_id":"proteintech-cl647-fca98449","title":"Proteintech, CL647-FcA98449, FcZero-rAb™ CoraLite® Plus 647 Anti-DYKDDDDK tag Rabbit Recombinant Antibody (Binds to FLAG® tag epitope)","description":"Size: 100ug\nThe DYKDDDDK tag (CL647-FcA98449) by Proteintech is a Recombinant antibody targeting DYKDDDDK tag in FC (Intra) applications with reactivity to n\/a samples\nCL647-FcA98449 targets DYKDDDDK tag in FC (Intra) applications and shows reactivity with n\/a samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive FC (Intra) detected in: Transfected HEK-293T cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nProtein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: n\/a\nHost \/ Isotype: Rabbit \/ IgG\nClass: Recombinant\nType: Antibody\nImmunogen: unknow Predict reactive species\nFull Name: DYKDDDDK tag\nConjugate: CoraLite® Plus 647 Fluorescent Dye\nExcitation\/Emission Maxima Wavelengths: 654 nm \/ 674 nm\nExcitation Laser: Red Laser (633 nm)\nForm: Liquid\nPurification Method: Protein A purification\nUNIPROT ID: FLAGTAG\nStorage Buffer: PBS with 0.09% sodium azide, pH 7.3.\nStorage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46888745861289,"sku":"CL647-FcA98449","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-cl647-fca98449","provider":"Iright","version":"1.0","type":"link"}