Proteintech, sms1AF488-1, ChromoTek Nano-Secondary® anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104]

Size: 10ul / 100ul Nano-Secondary® anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity. Specification Description: Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. Applications: IF, WB, FC Host: Alpaca Species Reactivity: Mouse IgG1 Fc-fragment No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG2a, IgG2b, IgG2c, IgG3 Target/Specificity: This Nanobody mixture recognizes the Fc fragment of mouse IgG1. Physical State: Liquid Conjugate: Alexa Fluor® 488 Suggested Dilution: Immunofluorescence 1:500 Super-resolution microscopy 1:500 Western blot 1:1,000 Purification Method: Recombinant expression, affinity purification IMAC Type: Mixture of 2 monoclonal Nanobodies; Secondary Nanobody Class: Recombinant Format: Alpaca single domain antibodies, monovalent Cross-reactivity: No cross-reactivity to rabbit, rat, sheep, goat, guinea pig, human, and macaque serum Immunogen: Purified mouse IgG1 Clonality: Biclonal: mixture of 2 monoclonal Nanobodies Clones: VHH0302, VHH0305 RRID: AB_2827579 Affinity (KD): CTK0103: K D =0.13 nM, CTK0104: K D = 0.63nM Excitation/ Emission: Excitation max: 490 nm, Emission max: 525 nm Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide Storage Condition: Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. Size: 10 µL; 100 µL Note: This product is for research use only, not for diagnostic or therapeutic use