{"product_id":"revvity-61pardaf","title":"Revvity, 61PARDAF, HTRF Anti-Rabbit IgG pAb d2-Conjugate, 1,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\n​IgGs from Goat anti-Rabbit immunoserum were immunopurified and labeled with d2. PAb Anti Rabbit IgG-d2 may be used to detect the binding of unlabeled Rabbit specific antibody on a target molecule or on Ig fusion proteins.\u003cbr\u003e\nThis reagent can be used in both biochemical and cellular formats to study a wide variety of interactions: protein\/protein, protein\/peptide, protein\/DNA, protein\/RNA, protein\/carbohydrate, protein\/small molecule, receptor\/ligand.\u003cbr\u003e\nHTRF can detect a broad range of affinity constants ranging from picomolar to low millimolar.\u003cbr\u003e\nHTRF assays offer many advantages over other technologies:\u003c\/p\u003e\n\n\u003cp\u003eHomogeneous add-and-read format\u003cbr\u003e\nNo wash steps\u003cbr\u003e\nLow background\u003cbr\u003e\nStraightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates\u003cbr\u003e\nStable signal, providing flexibility in time of readout or size of assays\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003cbr\u003e\n​In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners. In the example shown here: PAb Anti Rabbit IgG-d2 binds to the Rabbit Fc fused tagged partner A while partner B* binds to a specific Ab labeled with an HTRF donor. *partner B can also be biotinylated, tagged, Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with donor for the detection.\u003c\/p\u003e\n\n\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003c\/p\u003e\n\n\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nThe example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a Rabbit Fc fused-tagged partner A and a non-tagged partner B*. Dispense the 2 partners (10 µL), incubate, add PAb Anti Rabbit IgG-d2 (5 µL) and anti-partner B labeled with donor (5 µL), incubate and read.*partner B can also be biotinylated, tagged, Fc fused or directly labeled. In these cases, use the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin) labeled with donor for the detection. Application-Protein-Protein Interaction\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Fluorescent Reagent\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Biologics\u003cbr\u003e\nTarget Species-Rabbit\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Cardiovascular Infectious Diseases Inflammation Metabolism\/Diabetes NASH\/Fibrosis Neuroscience Oncology \u0026amp; Inflammation Rare Diseases\u003cbr\u003e\nUnit Size-1,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775299276969,"sku":"61PARDAF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/revvity-61pardaf","provider":"Iright","version":"1.0","type":"link"}