{"product_id":"revvity-64parpeh","title":"Revvity, 64PARPEH, HTRF Human and Mouse PARP cleaved-Asp214 Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nThe cleaved PARP (Asp214) kit enables the specific and quantitative detection of endogenous levels of the human, mouse, and monkey large fragment (89-kDa) of PARP-1. With its central position downstream from caspases 3 and 7, both activated by extrinsic and intrinsic apoptosis pathways, cleaved PARP-1 represents a pertinent marker of cells undergoing apoptosis. Deregulated apoptosis plays a role in various diseases involving insufficient cell death, such as cancer, autoimmunity, and persistent infections, whereas excessive apoptosis can contribute to neuro-degeneration and ischaemia.\u003cbr\u003e\nHow it works\u003cbr\u003e\nAssay principle\u003c\/p\u003e\n\n\n  \u003cbr\u003e\n        \n\n\n\t\u003cbr\u003e\n  \u003cbr\u003e\n        \u003cbr\u003e\n  \n\n      \u003cbr\u003e\n  \u003cbr\u003e\n  \u003cbr\u003e\n    \u003cbr\u003e\n      \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n        \u003cbr\u003e\n      \u003cbr\u003e\n    \n\n    \u003cbr\u003e\n      \n\n      \u003cbr\u003e\n        \n\n      \u003cbr\u003e\n    \n\n  \n\n\n\n\n\u003cp\u003e \u003cbr\u003e\nThe cleaved-PARP assay is a sandwich immunoassay using two specific anti-PARP-1 p85 fragment monoclonal antibodies, one labelled with Eu3+ Cryptate (donor) and the other with d2 (acceptor). Donor-acceptor proximity leads to a fluorescent TR-FRET signal proportional to the number of cells undergoing apoptosis. The protocol is optimized for a 384-well plate format, but can easily be further miniaturized or upscaled. After cell lysis, the cleaved PARP-1 p85 fragment can be semi-quantitatively detected using the HTRF cleaved-PARP-Asp214 assay kit reagents.\u003c\/p\u003e\n\n\n\u003cp\u003eAssay protocol\u003cbr\u003e\nTwo-plate assay protocol For added flexibility, the assay can be run under a two-plate assay protocol, where cells are plated and treated in a 96-well culture plate. For detection, lysates are subsequently transferred to a 384-well sv assay plate where the detection reagents are added. The detection reagents may be pre-mixed and added in a single dispensing step for direct detection. No washing is needed at any step. This also enables monitoring of the cells' viability and confluence in an appropriate cell culture plate. Application-Protein Quantification\u003cbr\u003e\nBrand-HTRF\u003cbr\u003e\nDetection Modality-HTRF\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-16 µL\u003cbr\u003e\nShipping Conditions-Shipped in Dry Ice\u003cbr\u003e\nTarget Class-Biomarkers\u003cbr\u003e\nTarget Species-Human Mouse\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Cardiovascular Infectious Diseases Metabolism\/Diabetes NASH\/Fibrosis Neuroscience Rare Diseases\u003cbr\u003e\nUnit Size-10,000 assay points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775299637417,"sku":"64PARPEH","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/revvity-64parpeh","provider":"Iright","version":"1.0","type":"link"}