BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Nkx2.2 564729

Alternative Name: NKX2-2; NKX2B; Nkx2-2; Nkx-2.2; NK-2 homolog B; glycoprotein GP330
Reactivity: Human (QC Testing), Mouse (Tested in Development), Rat, Chicken (Reported)
Isotype: Mouse BALB/c IgG2b, κ
Immunogen: Chicken Nkx2.2 Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging, Immunofluorescence (Tested During Development)
Concentration: 0.2 mg/ml
RRID: AB_2738922
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Recommended Assay Procedures: Recommended Assay Procedures Alexa Fluor® 647 Mouse Anti-Nkx2.2 effectively stains Nkx2.2-transfected 293-F cells when using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574). However, we recommend fixation with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilization with BD Phosflow™ Perm Buffer III (Cat. No. 558050) for staining of untransfected 293-F cells.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Triton is a trademark of the Dow Chemical Company. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.