Cytiva, 29032038, ÄKTA ready™ chromatography system

ÄKTA ready gradient The ÄKTA ready™ system family consists of a range of single-use liquid chromatography system options that are built for process scale-up and GMP manufacturing. The systems use disposable flow paths and prepacked columns to enable flexibility and speed in bioprocessing. • Fully disposable flow paths for gradient or isocratic configurations are quick to install and validate, improving productivity with quick campaign changeover. • Single-use flow paths mean no need for system cleaning validation, and minimized risk of cross-contamination between products/batches. • Well suited for clinical scale-up of monoclonal antibody (mAb) processes as well as for production of sensitive biologics such as viral vectors, nucleic acids, and antibody-drug conjugates (ADC’s). • Supports prepacked columns like ReadyToProcess™ columns packed with Cytiva chromatography resins. • For rate ranges from 3 L/h to 510 L/h with two sizes of flow kits on one platform. • Processes can be scaled from bench-scale ÄKTA ready™ 450 system to ÄKTA ready™ system and ultimately to ÄKTA ready™ XL system which can support purification of drug substance from 2000 L bioreactors. • Extensive product documentation and regulatory support is available to facilitate implementation in GMP environment. View data file | See the system in 3D (extended model) ÄKTA ready™ system is available in four standard configurations. The basic system is available in isocratic or gradient configuration with essential functionality and sensor capability for most common chromatography operations such as mAb and recombinant protein purifications. ÄKTA ready™ variable UV system builds on the ÄKTA ready™ gradient system by including a three-wavelength variable UV sensor (206-700 nm) and an integrated single-use pH electrode in the flow path to enable closed processing. These features support applications such as separation of full and empty adeno-associated virus (AAV) capsids and purification of nucleic acids. ÄKTA ready™ extended system builds further on the variable UV system by adding additional sensors and valves for enhanced functionality, an integrated human-machine interface (HMI), and capability to monitor and control Xcellerex™ XDUO mixers .

	ÄKTA ready™	ÄKTA ready™ variable UV	ÄKTA ready™ extended
	Isocratic	Gradient
Essential functionality	✓	✓	✓	✓
Gradient capability		One inlet	One inlet	Six inlets
Three variable UV wavelengths			✓	✓
Integrated pH electrode in flow kit			✓	✓
Pre-column conductivity and air sensors				✓
Automated air trap				✓
Up/down flow on column				✓
Integrated HMI				✓

See more comparisons of these systems. Flow kits and prepacked columns for ÄKTA ready™ systems Flow kits: The Low flow kit supports flow rates 3-175 L/h for columns typically less than 20 cm inner diameter (approximately 5 L volume). The High flow kit supports flow rates 7.5-510 L/h for columns with diameter more than 20 cm (approximately 10 L or larger). Gradient flow kit sections are available separately. Flow kits are double-bagged and gamma-irradiated before delivery. View the flow kit products Prepacked columns: ÄKTA ready™ systems support prepacked columns like ReadyToProcess™ columns as a complete single-use downstream purification package. The validated columns can be set up in minutes, removing the need for column packing, column verification, and other time-intensive steps. ÄKTA ready™ system supports column sizes from approximately 1 L to 20 L volume. View options for ReadyToProcess™ columns Expanded control software options You now have more options to fit your automation needs. UNICORN™ software is designed according to GAMP 5 guidelines and can be used in a manner that follows 21 CFR part 11 requirements. UNICORN™ software displays key run data, and allows the user to set and view manual instructions and alarm limits for components. Connecting flow kits is guided by an installation wizard on the basic ÄKTA ready™ system, and ReadytoProcess™ columns can be set up in minutes using the predefined options in the column list. For users who require chromatography equipment to be used on their facility’s plant-wide automation platform, ÄKTA ready™ system can be customized with a distributed control system or can connect to automation options via Open Platform Communications-Unified Architecture (OPC-UA). Visit the UNICORN™ software page .