Product Description
Laminin is the major glycoprotein component of basement membranes and has a molecular weight of 850,000 daltons. This preparation of mouse Laminin was isolated from the Engelbreth-Holm-Swarm (EHS) sarcoma and is supplied at a concentration of 0.5-2.0 mg⁄ml.
Key functions include:
• Modulation of cell attachment, cell spreading, cell growth, and motility
• Binding to itself and other matrix compounds
• Promotion of epithelial differentiation
• Modification of leukocyte function
• Stimulation of neurite outgrowth
Supplied: 1 mg in 50 mM Tris-HCL (pH 7.4), 0.15 M NaCl
Performance qualification (cell attachment): Half-maximal cell attachment of MG63 cells at 37°C: ≤ 20 ug⁄ml
Key functions include:
• Modulation of cell attachment, cell spreading, cell growth, and motility
• Binding to itself and other matrix compounds
• Promotion of epithelial differentiation
• Modification of leukocyte function
• Stimulation of neurite outgrowth
Supplied: 1 mg in 50 mM Tris-HCL (pH 7.4), 0.15 M NaCl
Performance qualification (cell attachment): Half-maximal cell attachment of MG63 cells at 37°C: ≤ 20 ug⁄ml
FAQs
1. Can I use the Poly-D-Lysine matrix with other coatings?
Yes. iPSC-derived neurons have been successfully grown on Poly-D-Lysine followed by an additional coating step. A common approach is to apply mouse or human laminin as a secondary coating on top of the Poly-D-Lysine layer.
2. Can natural mouse laminin be thawed, aliquoted, and refrozen?
Yes. It’s recommended to divide the product into single-use (or working-size) aliquots after thawing and store them at -68°C to -85°C to minimize repeated freeze–thaw cycles.
3. What is the protein concentration per vial for natural mouse laminin?
Each vial contains 1 mg of laminin. The protein concentration for a given lot may fall within 0.5–2.0 mg/mL, so the fill volume can vary from vial to vial depending on the measured concentration for that lot. Regardless of volume, the amount per vial is adjusted to total 1 mg of laminin.
Order Guidelines
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3. Minimum order value of $1,000 USD required.
Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924