Waters, 176003714, GlycoWorks RapiFluor-MS N-Glycan Refill Kit-24 sample

Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive. This refill kit includes the GlycoWorks Deglycosylation Module (186008841) and the GlycoWorks Labeling Module (186008091) shipped together in order to supplement either the 176003712 or 176003713 kits or as extra reagents for the 96 sample kits. It contains materials to process 24 samples in a 3x8 format. The kit contains: GlycoWorks Deglycosylation Module: (1) 0.035 ml/vial Rapid PNGaseF Enzyme (1) 0.25 ml/vial GlycoWorks Rapid Buffer (3) 3 vials of 3 mg/vial RapiGest™ SF Surfactant GlycoWorks RapiFluor-MS Labeling Module: (3) 9 mg/vial RapiFluor-MS Reagent (3) 1 ml/vial Reagent Solvent (Anhydrous DMF)

Specifications
Number of Analysis: 24
Type: Sample Preparation Workflow
Packages per kit: 2
Separation Mode: HILIC
Detection Method: MS, MS/MS, UV, HRMS
Storage Condition: Refer to the storage recommendations of each sub-package
System Type: LC-MS
Shelf Life: Refer to the storage recommendations of each sub-package
UNSPSC: 41116104
Application: Glycan
Brand: GlycoWorks
Product Type: Application Kits
Units per Package: 1 pk

Additional Information
GlycoWorks RapiFluor-MS N-Glycan Refill Kit-24 sample Analyzing released N-glycans doesn’t have to be time-consuming and complicated. The GlycoWorks RapiFluor-MS N-Glycan Kit allows you to follow an easy 3 step protocol that can be completed in as little as 30 minutes, allowing for sample preparation that is more efficient and enhances productivity. The unique chemical attributes in RapiFluor-MS allow for increased fluorescence quantification and supreme mass spectral response. Using a single label to gain valuable information ranging from characterization to routine monitoring, Glycan analysis has been made easier and more sensitive with GlycoWorks RapiFluor-MS N-Glycan Refill Kits. This innovative sample preparation method makes use of optimized deglycosylation conditions and reagents to enable a fast release. The novel reagents included were purposefully designed to provide the benefits of both sensitive fluorescence detection and superior matching signal intensity for mass detection. Before liquid chromatography analyses, sample clean-up is accomplished using solid-phase extraction with a GlycoWorks HILIC µElution Plate. This refill kit includes materials to process 24 samples in a 3x8 format and comes with GlycoWorks Deglycosylation Module and the GlycoWorks Labeling Module shipped together to supplement other kits or as reagents for GlycoWorks RapiFluor-MS N-Glycan Kit- 96 Sample . Additionally, this kit includes GlycoWorks Deglycosylation Module (1), 0.035 ml/vial Rapid PNGaseF Enzyme (1), 0.25 ml/vial GlycoWorks Rapid Buffer (3), 3 vials of 3 mg/vial RapiGest SF Surfactant, GlycoWorks RapiFluor-MS Labeling Module (3), 9 mg/vial RapiFluor-MS Reagent (3), 1 ml/vial Reagent Solvent (Anhydrous DMF). All lab equipment is manufactured to industry and regulatory standards, with the appropriate vessels for each substance being used for packaging.

FAQ
How Should I Equilibrate My Columns Before Use With RapiFluor-MS? Before getting started with Glycan analysis, a newly received Glycan BEH Amide Column should be flushed in 60% acetonitrile: 40% aqueous for 50 column volumes. Equilibrate with 20 column volumes of initial mobile phase conditions before making the first injection. Between injections, equilibrate with 8-10 column volumes. Failure to appropriately follow these steps to equilibrate the column may lead to drifting retention times. What Is The Three-Step Protocol for Sample Preparation? The simple three-step process enabled by the GlycoWorks RapiFluor-MS N-Glycan Refill Kit is as follows.
Step 1: Deglycosylation, which takes less than 15 minutes.
Step 2: Labeling, expected to take 5 minutes.
Step 3: Clean up, possible to complete within 10 minutes.