Agilent, 5065-9924, ZORBAX 300SB-C18, 3.5 µm, 0.075 x 50 mm

ZORBAX 300SB-C18, 3.5 µm, 0.075 x 50 mm. A reversed-phase C18 HPLC column with fully porous ZORBAX particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: ZORBAX 300SB-C18
Hardware: SS
Inner Diameter (ID): 0.075 mm
LC Platform: Stainless Steel
Length: 50 mm
Maximum Temperature: 90 °C
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: 300SB-C18
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 1-8

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The Agilent ZORBAX 300SB-C18 column offers robust performance for high-sensitivity liquid chromatography applications, particularly in proteomics and peptide mapping. Featuring a 3.5-micron particle size and a narrow-bore dimension of 0.075 x 50 millimeters, this reversed-phase column delivers excellent resolution and efficient separations with minimal sample consumption, making it ideal for limited or precious biological samples. The StableBond C18 chemistry ensures outstanding stability at low pH, supporting reproducible results across a wide variety of complex biological matrices.

Optimized for nano-flow LC-MS workflows, this column is compatible with advanced Agilent liquid chromatography systems, such as the 1290 Infinity, 1260 Infinity, and compatible nano LC-MS platforms from other major manufacturers. It is designed for critical research applications including protein and peptide characterization, biomarker discovery, and quantitative proteomics. The column’s high peak capacity and mechanical strength make it suitable for demanding analytical laboratories aiming for precise and reliable performance.

For comprehensive workflows, the column integrates seamlessly with other ZORBAX 300SB or Rapid Resolution columns for multidimensional separations. Its durability and batch-to-batch consistency ensure dependable results, enabling researchers to streamline method development and accelerate discoveries in life science and biopharmaceutical research.