Agilent, 5190-2452, Agilent Bio WCX, NP5, 2.1 x 50 mm, PEEK

Agilent Bio WCX, NP5, 2.1 x 50 mm, PEEK. A weak cation exchange HPLC column with nonporous particles. Particle size is 5 μm. PEEK hardware enables this column to couple with bio-compatible or bio-inert LC systems.

Specifications:
Brand: Agilent Bio WCX
Column Material: PEEK
Guard Column: No
Hardware: PEEK
Inner Diameter (ID): 2.1 mm
LC Platform: BioInert
Length: 50 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WCX, NP5, 2.1 x 50 mm, PEEK column is designed for high-performance weak cation exchange chromatography in biomolecular research. Featuring a 2.1 mm internal diameter and a 50 mm length, this column utilizes a non-porous 5 µm particle (NP5) size, delivering rapid and efficient separation of proteins, peptides, and other charged biomolecules. The PEEK (polyether ether ketone) hardware provides excellent chemical resistance and minimizes interaction with sample components, making it highly suitable for bioanalytical workflows where sample integrity is crucial. Optimized for reproducibility and robustness, this column supports applications such as protein isoform profiling, antibody analysis, and the characterization of protein charge variants in biopharmaceutical development. Compatible with a wide range of liquid chromatography systems, including Agilent 1100 and 1200 Series, this column integrates seamlessly into existing analytical setups. It is an ideal choice when high-resolution, reproducible separations are required under demanding analytical conditions. Commonly paired with Agilent’s range of LC detectors and sample preparation products, this column enables precise and reliable analysis for research and quality control settings in pharmaceutical, biotechnology, and academic laboratories.