Agilent, 5190-2523, Agilent Bio SEC-5, 150Å, 4.6 x 300 mm, 5 µm

Agilent Bio SEC-5, 150Å, 4.6 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.5-150 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 500 Da
Molecular Weight Upper Limit: 150000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 150 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 150 Angstrom column is a high-performance size exclusion chromatography column designed for precise molecular size analysis of proteins, peptides, and other biomolecules. With a dimension of 4.6 x 300 mm and a 5 micron particle size, this column offers excellent resolution and consistent performance in analytical and quality control settings. The 150 Angstrom pore size is particularly suitable for separating low to medium molecular weight biomolecules, making it ideal for applications in protein purification, biopharmaceutical characterization, and aggregate analysis.

This column is constructed with robust silica and a stable bonded phase, ensuring durability and reproducibility during routine laboratory workflows. It is compatible with a wide range of aqueous mobile phases, supporting sensitive detection by UV, refractive index, or light scattering detectors. The Bio SEC-5 column seamlessly integrates with Agilent HPLC and UHPLC systems, as well as other standard liquid chromatography instruments. It pairs effectively with Agilent’s molecular weight calibration standards and related SEC columns, providing reliable benchmarking and method flexibility. Researchers and analysts trust this column for its accuracy in molecular sizing, critical for method development, quality assurance, and regulated environments in biopharma and life science research.