Agilent, 5190-6872, Agilent Bio SEC-5, 500A, 21.2 x 50 mm, 5 µm

Agilent Bio SEC-5, 500A, 21.2 x 50 mm, 5 µm. A preparative scale size exclusion column for the purification of large biological samples such as adeno associated virus. Resolving range 15 kDa - 5 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 50 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 15000 Da
Molecular Weight Upper Limit: 5000000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 500 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-5 column with a 500A pore size, 21.2 x 50 mm dimensions, and 5 micron particle size delivers robust and reliable size exclusion chromatography for biomolecular analysis. Engineered with proprietary silica and specialized bonding, the column provides high resolution separation of proteins, antibodies, and other biological macromolecules across a wide molecular weight range. Its 500A pore size optimizes the analysis of large biomolecules, making it an excellent choice for characterizing aggregate content, determining molecular weight distribution, and purifying preparative samples for downstream applications. This column is ideal for use in protein characterization, quality control, and biotherapeutic development workflows. It is fully compatible with Agilent and other major HPLC and UHPLC systems, offering seamless integration into existing laboratory setups. Recommended use includes protein aggregate monitoring, analysis of monoclonal antibodies, and assessment of biosimilar comparability. For comprehensive biomolecule analysis, the product can be paired with Agilent Bio SEC standard columns and detectors such as refractive index or diode array detectors. Designed for high throughput and reproducibility, this size exclusion column ensures consistent, accurate results to meet the critical demands of biopharmaceutical research and production laboratories.