Agilent, 763750-902, ZORBAX Extend 300 C18, 2.1 x 150 mm, 3.5 µm

ZORBAX Extend 300 C18, 2.1 x 150 mm, 3.5 µm. A reversed-phase C18 HPLC column with fully porous ZORBAX particles. The 300-Extend C18 chemistry incorporates a unique bidentate silane which allows the column to withstand pH up to 11.5.

Specifications:
Brand: ZORBAX
Hardware: SS
Inner Diameter (ID): 2.1 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 40 °C at pH 8-11.560 °C at pH 6-8
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: Extend-C18
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 2-11

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The ZORBAX Extend 300 C18 column, featuring a 2.1 x 150 mm dimension and 3.5 micron particle size, is designed for high-performance liquid chromatography requiring robust reversed-phase separations. Engineered with a stable, extended-pH silica and a 300 angstrom pore size, this column enables efficient analysis of large biomolecules, such as peptides, protein fragments, and oligonucleotides, while also supporting traditional small molecule separations. Its wide pH operating range (2-11.5) allows for method flexibility and enhanced analyte resolution, particularly in applications necessitating higher pH mobile phases. Typical research uses include proteomic studies, peptide mapping, biopharmaceutical characterization, and impurity profiling. The column’s optimized particle size and pore structure deliver high efficiency and sharp peak shapes for complex samples. It is fully compatible with Agilent HPLC and UHPLC systems, including 1100, 1200, 1260, and 1290 Infinity Series instruments, and can be integrated into workflows using other manufacturers’ systems with standard column hardware. For broader analytical capabilities, the column is part of Agilent’s ZORBAX Extend family, which includes a range of pore sizes and chemistries for diverse separation needs in pharmaceutical, biotechnological, and academic research laboratories.