Agilent, GP24NG-LB, GlykoPrep Rapid N-glycan Prep with InstantAB, 24-ct

GlykoPrep Rapid N-glycan Prep with InstantAB, 24-ct. Complete kit. GlykoPrep is a cartridge based workflow with rapid digestion, instant labeling, and cleanup of excess InstantAB label. Includes GS24-RX digestion module, GS24-LB InstantAB labeling module, GS24-CU cleanup module.

Specifications:
Application: Flexible formatNonselective, rapid release and recovery of intact N-glycansPurified, fluorescently labeled N-glycans are produced in water and ready for analysis without concentrating or dryingRT labeling with InstantAB with reduced loss of sialic acid compared to reductive amination labeling chemistrylabeling efficiency >80%
Count: 24
Equipment & Reagents Provided by User: Acetonitrile (ACN, 100%, HPLC-grade)AssayMAP Labware (AM200)Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm heightFormic acid (HPLC-grade)Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)Ultrapure, deionized water
Kit Configuration: Sample Prep Kit
Label: InstantAB
Prep time (approx): 3 hours
Technique: LC

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This rapid N-glycan preparation kit provides a streamlined solution for efficient deglycosylation and fluorescent labeling of N-linked glycans from glycoproteins. Using InstantAB dye chemistry, the system enables sensitive, high-throughput sample processing in under two hours, accommodating up to 24 samples per kit. The protocol integrates enzymatic protein digestion and rapid labeling steps, minimizing hands-on time while delivering reliable and consistent results.

Optimized for both novice and experienced users, this kit is suitable for a variety of research scenarios including biopharmaceutical development, glycomics studies, and quality control analyses. The labeled glycans generated are fully compatible with liquid chromatography and mass spectrometry platforms, such as the Agilent InfinityLab LC series and Agilent 6200 Series TOF/6545 Q-TOF systems. Additionally, it supports downstream applications in glycan profiling, biomarker discovery, and batch-to-batch comparability assessments.

For enhanced data analysis, the workflow aligns with Agilent Glycan Analysis Software and can be paired with Glyko standards and consumables for expanded experimental flexibility. This comprehensive kit delivers reproducibility, sensitivity, and speed, facilitating confident glycoprofiling for both academic and industrial environments.