Agilent, GP96NG-LB, GlykoPrep Rapid N-glycan Prep with InstantAB, 96-ct

GlykoPrep Rapid N-glycan Prep with InstantAB, 96-ct. Complete kit. GlykoPrep is a cartridge based workflow with rapid digestion, instant labeling, and cleanup of excess InstantAB label. Includes GS24-RX digestion module, GS24-LB InstantAB labeling module, GS24-CU cleanup module.

Specifications:
Application: Flexible formatNonselective, rapid release and recovery of intact N-glycansPurified, fluorescently labeled N-glycans are produced in water and ready for analysis without concentrating or dryingRT labeling with InstantAB with reduced loss of sialic acid compared to reductive amination labeling chemistrylabeling efficiency >80%
Count: 96
Equipment & Reagents Provided by User: Acetonitrile (ACN, 100%, HPLC-grade)AssayMAP Labware (AM200)Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm heightFormic acid (HPLC-grade)Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)Ultrapure, deionized water
Kit Configuration: Sample Prep Kit
Label: InstantAB
Prep time (approx): 3 hours
Technique: LC

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This advanced kit streamlines the release, labeling, and purification of N-linked glycans from glycoproteins, utilizing efficient enzymatic digestion and rapid fluorophore conjugation with InstantAB dye. Supplied in a convenient 96-count plate format, it enables high-throughput sample processing suitable for biopharmaceutical development, glycomics research, and analytical quality control of therapeutic proteins. The robust workflow delivers reliable results within hours, supporting both manual and automated protocols. Purified, fluorescently labeled glycans are compatible with a wide range of downstream analyses, including HPLC, UHPLC, and CE-LIF platforms, as well as mass spectrometry methods when paired with appropriate detection modules. This product supports detailed glycan profiling and characterization, aiding identification of structural variants and ensuring regulatory compliance in bioprocessing pipelines. Ideally paired with Agilent LC and CE instruments, the workflow is also adaptable for integration with other leading analytical systems and sample handling solutions. Related reagents and consumables for glycan analysis, like GlykoPrep O-glycan kits and standards, are also available to complement the full suite of glycomic tools, enabling comprehensive characterization for critical quality attributes in monoclonal antibody and glycoprotein research.