Agilent, GX96-2AB, AdvanceBio Gly-X N-glycan prep with 2-AB Express kit, 96-ct

AdvanceBio Gly-X N-glycan prep with 2-AB Express kit, 96-ct. Complete kit. utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 2-AB dye. With deglycosylation carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the 2-AB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX96-100 Gly-X deglycosylation module (96-ct), GX96-401 Gly-X 2-AB Express labeling module (96-ct), GX96-402 Gly-X 2-AB Express cleanup module (96-ct).

Specifications:
Application: 5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan releaseFlexible, high-throughput formatProcess 1 to 96 samplesOptimized cleanup removes excess free dye, protein, and other interfering compounds.Reductive amination 2-AB labeling chemistry without dry down
Count: 96
Deglycosylation Efficiency: > 99 %
Equipment & Reagents Provided by User: 96-well Thermocycler or two independent heat blocksAcetonitrile (ACN), MS-grade (Fisher A955-4 recommended)DI waterThermocycler (Corning THERM-1001, 110V, THERM-1000, 230V)Vacuum manifold (Millipore MSVMHTS00-HTS)Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB starter kit - included free of charge with first Gly-X order, please contact us for details)Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522)
Kit Configuration: Sample Prep Kit
Label: 2-AB
Prep time (approx): ~2.5 hours
Preservation of Sialylated Species: > 99 %
Protein Load Range: 1-40 µg
Recovery: > 80 %
Stability: At least 1 month at -20°C, 5 days at 4–8°C
Technique: LC & LC/MS
UNSPSC Code: 41115711

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The AdvanceBio Gly-X N-glycan prep with 2-AB Express kit enables rapid, streamlined preparation and labeling of N-glycans from biopharmaceutical and glycoprotein samples for high-throughput analysis. This 96-count kit utilizes advanced enzymatic deglycosylation and efficient 2-aminobenzamide (2-AB) fluorescent labeling technology for sensitive glycan detection and quantitation. Designed for robust performance, the workflow supports sample processing in under three hours, reducing hands-on time and minimizing variability. It is ideal for research applications such as glycoprofiling, biotherapeutic characterization, quality control, and biosimilarity assessments by supporting both discovery and routine analysis. The kit is fully compatible with Agilent HPLC and LC-MS systems, as well as other major platforms, enabling clear, reproducible results with minimal background. Related products include other AdvanceBio Gly-X kits for alternate labeling chemistries and optimized glycan columns for comprehensive separation. With its high-throughput format, reproducible performance, and compatibility with automated liquid handling, this kit is an excellent choice for researchers and QC laboratories seeking efficient, reliable N-glycan preparation and analysis workflows in biopharmaceutical development and quality assurance settings.