Agilent, GX96-402, AdvanceBio Gly-X 2-AB Express cleanup module, 96-ct

AdvanceBio Gly-X 2-AB Express cleanup module, 96-ct. Module for use with Gly-X N-glycan prep and labeling with 2-AB Express modules. This cleanup plate is used for on-matrix 2-AB labeling as well as cleanup of free dye.

Specifications:
Application: Optimized cleanup removes excess free dye, protein, and other interfering compounds.
Count: 96
Deglycosylation Efficiency: > 99 %
Equipment & Reagents Provided by User: Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)DI waterGly-X deglycosylation and 2-AB Express labeling 24-ct [GX24-401AB] or 96-ct [GX96-401AB]ProZyme vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB starter kit, included free of charge with first Gly-X order, please contact us for details)Vacuum manifold (Millipore MSVMHTS00-HTS)Vacuum pump (Millipore WP6211560, 110V. WP6122050, 220V. Welch WOB-L Pump 2522)
Kit Configuration: Sample Prep Module
Label: 2-AB
Precision (well-to-well, day-to-day): <5% CV
Prep time (approx): 130 min (24 samples)
Preservation of Sialylated Species: > 99 %
Protein Load Range: 1-40 µg
Recovery: > 80 %
Stability: At least 1 month at -20°C, 5 days at 4–8°C
Technique: LC & LC/MS

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The AdvanceBio Gly-X 2-AB Express cleanup module enables rapid and efficient purification of fluorescently labeled N-glycans from complex biological samples. Designed for 96-well format, this kit streamlines and standardizes sample preparation workflows, supporting high-throughput glycan analysis required in biopharmaceutical and glycomics research. By removing excess labeling reagents, salts, and other contaminants, the module ensures optimal sample quality for downstream analysis.

Researchers employing techniques such as HPLC, UHPLC, or LC-MS for glycan profiling will benefit from improved sensitivity, reproducibility, and data accuracy. The module is fully compatible with Agilent AdvanceBio Gly-X labeling kits and seamlessly integrates with a wide range of Agilent HPLC and LC-MS systems, as well as other standard analytical platforms. This allows for flexible implementation in both routine and advanced glycan characterization studies.

Typical usage scenarios include quality control and characterization of therapeutic proteins such as monoclonal antibodies, vaccine development, and biomarker discovery. With pre-packed plates and a simplified protocol, the module minimizes hands-on time and ensures consistent, reliable cleanup. This product supports efficient sample throughput, making it an ideal choice for laboratories needing robust, reproducible, and scalable glycan cleanup solutions.