Agilent, PL1580-5350, AdvanceBio SEC 130Å, 4.6 x 300 mm, 2.7 µm, LC column

AdvanceBio SEC 130Å, 4.6 x 300 mm, 2.7 µm, LC column. A size exclusion column for efficient aggregate analysis of peptides and small proteins. The resolving range is 0.1-120 kDa.

Specifications:
Brand: AdvanceBio SEC
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 120000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 2.7 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 130 Å
Pressure Rating: 400 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: 0.02% Sodium Azide
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The AdvanceBio SEC 130Å, 4.6 x 300 mm, 2.7 µm LC column from Agilent is designed for high-resolution size exclusion chromatography of biomolecules, particularly protein and biotherapeutic samples. With a pore size of 130 angstroms and sub-3 micron particle technology, this column delivers superior resolution and rapid, reliable separation of monomers, aggregates, and fragments. It is ideal for applications such as aggregate analysis, molecular weight determination, and characterization of antibodies or other biopharmaceutical proteins in research or quality control laboratories.

The robust silica-based packing ensures consistent performance and long column life even with demanding samples. With its 4.6 mm internal diameter and 300 mm column length, it offers optimal flow and sample capacity for both analytical and semi-preparative workflows. Compatible with a wide range of liquid chromatography systems, it is particularly recommended for use with Agilent InfinityLab LC Series, Agilent 1260, and 1290 Infinity or comparable UHPLC platforms.

This column integrates seamlessly with related Agilent AdvanceBio SEC offerings and standard SEC mobile phases, supporting regulatory compliance and method transfer between laboratories. It is an excellent choice for scientists focused on reliable, reproducible biomolecule characterization.