BD Simultest™ Anti-Human CD57 FITC/CD8 PE 349510

Reactivity: Human
Application: Flow cytometry
Regulatory Status: RUO (GMP)
RRID: AB_400409
Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.
Description: Description CD57 (Leu-7): Clone HNK-1 is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with membrane extracts of the HSB-2T-lymphoblastoid cell line. CD8 (Leu-2a): Clone SK1 is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood T lymphocytes. The CD57 (Leu-7) antigen is a human lymphocyte antigen,  Mr 110 kdaltons (kDa), that is a carbohydrate structure associated with myelin-associated glycoprotein (MAG). The CD8 (Leu-2a) antigen is expressed on the 32-kDa α-subunit of a disulfide-linked bimolecular complex. The CD8 antigen binds to Class I major histocompatibility complex (MHC) molecules on antigen-presenting cells (APC), resulting in increased adhesion between the CD8 + T lymphocytes and the APCs. Binding of the CD8 antigen to Class I MHC molecules enhances the activation of resting T lymphocytes. The CD8 antigen is coupled to a protein tyrosine kinase, p56 lck . The CD8:p56 lck complex may play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3 antigen/T-cell antigen receptor (TCR) complex.
Preparation And Storage: Preparation And Storage The Simultest reagent is supplied as a combination of CD57 (Leu-7) FITC and CD8 (Leu-2a) PE in 1.0 mL of phosphate-buffered saline. Buffered saline contains gelatin and 0.1% sodium azide. The vial should be stored at 2° to 8°C and protected from prolonged exposure to light. Simultest reagents should not be frozen. Each Simultest reagent is stable for the period shown on the bottle label when stored as directed.
Description : Clone : Isotype : EntrezGene ID
N/A : N/A : | IgM, κ,IgG1, κ : N/A