BD, 550298, BD Pharmingen™ Purified Mouse Anti-Rat CD8a

Alternative Name: Cd8a; CD8α; CD8 alpha; OX-8 membrane antigen
Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: High-molecular-weight rat thymocyte glycoproteins
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed (Tested During Development), Blocking, Immunoaffinity Chromatography, Immunoprecipitation, Western blot (Reported)
Concentration: 15.625 µg/ml
RRID: AB_2075364
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The OX-8 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections and paraffin sections. For paraffin sections no pretreatment is required. Tissues tested were rat spleen and thymus. The antibody stains the CD8 subset of T lymphocytes. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, the OX-8 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated polyclonal anti-mouse Ig (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the anti-mouse Ig HRP detection kit (Cat. No. 551011) that contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate can be used for staining.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).