BD, 550390, BD Pharmingen™ Purified Mouse Anti-Human CD34

Alternative Name: gp105-120; My10; Hematopoietic progenitor cell antigen CD34
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
Concentration: 250 µg/ml
Workshop Number: V MA27, VI E004
RRID: AB_393656
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: For optimal indirect immunohistochemical staining, investigators are encouraged to titrate the Mouse Anti-Human CD34 antibody (clone 581) (suggested starting dilution of 1:10 to 1:50) and visualize staining via a three-step staining procedure in combination with a Biotin Goat Anti-Mouse Ig (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) using a DAB detection system (Cat. No. 550880).  Alternatively, investigators may want to consider using the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011), which contains the biotinylated secondary antibody, antibody diluent, Streptavidin-HRP and DAB substrate.