BD, 550529, BD Pharmingen™ Biotin Rat Anti-Mouse CD25

Alternative Name: IL-2 Receptor α chain, p55
Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgM, κ
Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 125 µg/ml
RRID: AB_2125455
Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The 7D4 antibody specific for mouse CD25 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen and thymus. The antibody stains activated T and B lymphocytes and immature thymocytes. The isotype control recommended for use with this antibody is Biotin Rat IgM, κ Isotype Control (Cat. No. 550343). For optimal indirect immunohistochemical staining, the biotin format of the 7D4 antibody should be titrated (1:10 to 1:50 dilution) and visualized by Streptavidin HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure can  be found on our website at http://www.bdbiosciences.com/us/s/resources. The clone 7D4 is not recommended for form alin-fixed paraffin embedded sections.