BD, 551853, BD Pharmingen™ Purified Rat Anti-Mouse CD124

Alternative Name: Il4r; IL-4Rα; Il4ra; IL-4RA; IL-4R-alpha; IL4-BP; IL-4 Receptor α chain
Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgG2a, κ
Immunogen: Mouse CTLL-19.4 T Cell Line
Application: Flow cytometry (Routinely Tested), ELISA (Tested During Development), Immunoprecipitation, Neutralization (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394274
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The purified form of the mIL4R-M1 antibody can be used for the immunofluorescent staining (≤ 1 µg antibody/10e6 cells) and flow cytometric analysis of nucleated mouse cells to measure their expressed levels of surface CD124. An appropriate purified Ig isotype control is clone R35-95 (Cat. No. 553927). A three-layer staining protocol is recommended for maximizing the detection of IL-4Rα expressed by cells as detailed in the figure legend. ELISA: The purified mIL4R-M1 antibody is useful for a sandwich ELISA that measures soluble mouse CD124 protein levels. The biotinylated mIL-4R-M1 antibody (Cat. No. 552508) can be paired with the purified mIL4R-M2 (rat anti-mouse sIL-4R) antibody, Cat. No. 552952, and recombinant soluble mIL-4Rα as the standard. Neutralization: The mIL4R-M1 antibody reportedly blocks IL-4's binding to and biological effects on IL-4R-positive cells. Note: for use in in vivo and in vitro systems, our NA/LE (no azide, low endotoxin) format, Cat. No. 552288 is recommended. Immunoprecipitation: The mIL4R-M1 antibody is reported to immunoprecipitate mouse IL-4R proteins. Please note that this application is not routinely tested at BD Biosciences Pharmingen.