BD, 551974, BD Pharmingen™ PE Mouse Anti-Mouse CD212

Alternative Name: Il12rb1; IL-12R-beta-1; IL-12 Receptor β1 chain
Reactivity: Mouse (QC Testing)
Isotype: Mouse IgG2a, κ
Immunogen: IL-12Rβ1 Transfected Cell Line
Application: Flow cytometry/immunoassay (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_394310
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Recommended Assay Procedure: Immunofluorescent Staining and Flow Cytometric Analysis. The R-PE-conjugated 114 antibody (Cat. No. 551974) can be used for the immunofluorescent staining (≤ 1 µg antibody/10e6 cells) and flow cytometric analysis of muose T cells and NK cells to measure their expressed levels of surface IL-12Rβ1. An appropriate PE-conjugated immunoglobulin isotype control is clone G155-178 (Cat. No. 553457).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.